Insertion junction: LMJ.RY0402.237333_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.237333
Chromosome:chromosome 7
Location:1587529
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g324800 SNE6 (1 of 8) PTHR10366//PTHR10366:SF411 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // HIGH CHLOROPHYLL FLUORESCENCE PHENOTYPE 173 PROTEIN; Cinnamoyl-CoA reductase/flavanone 4-reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCCACATCCTGCAATACGTCCGAAGCCT
Internal bar code:CACGAGCTCAATTCGGGCTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:954
LEAP-Seq percent confirming:99.3548
LEAP-Seq n confirming:11549
LEAP-Seq n nonconfirming:75
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CATCCCTAAACTCTCGCTGC
Suggested primer 2:TGCCATGTGGATGACGTAGT