Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.237339 |
Chromosome: | chromosome 7 |
Location: | 2058888 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g325812 | MAW1,ZYS2 | Membrane-associated hydroxyproline-rich glycoprotein 1; (1 of 80) IPR003882 - Pistil-specific extensin-like protein | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCATCCATCTCTATGGCCATGCGCAACG |
Internal bar code: | CTGTGTCCAGGGCCGTGGTATC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 509 |
LEAP-Seq percent confirming: | 98.024 |
LEAP-Seq n confirming: | 9475 |
LEAP-Seq n nonconfirming: | 191 |
LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGTGGCAAGAAGAAGTGAGC |
Suggested primer 2: | TAGGTGGAGATGGGGATGTT |