| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.237354 |
| Chromosome: | chromosome 16 |
| Location: | 4762610 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686600 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATGCGAGTTTGCTGACGTGCACCAACCT |
| Internal bar code: | TGGTTCGTTTCCATACCACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 663 |
| LEAP-Seq percent confirming: | 98.1825 |
| LEAP-Seq n confirming: | 7779 |
| LEAP-Seq n nonconfirming: | 144 |
| LEAP-Seq n unique pos: | 118 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCTTGTCGTGAGTCTGGT |
| Suggested primer 2: | CCTCTTGCTGCCTACGAAAC |