| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.237366 |
| Chromosome: | chromosome 5 |
| Location: | 1292042 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245101 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCGTTGGGCCCGGGCACACACACACAC |
| Internal bar code: | GTCGACGACCGGCTGAAAACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 148 |
| LEAP-Seq percent confirming: | 55.5508 |
| LEAP-Seq n confirming: | 1311 |
| LEAP-Seq n nonconfirming: | 1049 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGGGAGTAAGCCGTTCAT |
| Suggested primer 2: | GAGGGGAAAGGTACATGGGT |