| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.237410 |
| Chromosome: | chromosome 7 |
| Location: | 2419300 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g328850 | CNK8 | NimA-related protein kinase; (1 of 1) PTHR24361:SF240 - PROTEIN MTK-1, ISOFORM B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCGGAAGCACCACAGCCCTCTGCTGTTT |
| Internal bar code: | GAATCATGACCGGAAAAAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 665 |
| LEAP-Seq percent confirming: | 98.8932 |
| LEAP-Seq n confirming: | 2770 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCAAAGAAAGGTGGCTG |
| Suggested primer 2: | CACAAGTATGCCCTGGGAGT |