Insertion junction: LMJ.RY0402.237410_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.237410
Chromosome:chromosome 7
Location:2419331
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g328850 CNK8 NimA-related protein kinase; (1 of 1) PTHR24361:SF240 - PROTEIN MTK-1, ISOFORM B 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAACAGCCCAACCACACACACCAAACAA
Internal bar code:TACATGGGACGATCTCTTAACA

Confirmation - LEAP-Seq

LEAP-Seq distance:785
LEAP-Seq percent confirming:99.4775
LEAP-Seq n confirming:7045
LEAP-Seq n nonconfirming:37
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGCAAAGAAAGGTGGCTG
Suggested primer 2:CACAAGTATGCCCTGGGAGT