| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.237435 |
| Chromosome: | chromosome 12 |
| Location: | 5085466 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g526650 | (1 of 8) IPR000719//IPR002290//IPR011009//IPR020635//IPR020636 - Protein kinase domain // Serine/threonine/dual specificity protein kinase, catalytic domain // Protein kinase-like domain // Tyrosine-protein kinase, catalytic domain // Calcium/calmodulin-dependent/calcium-dependent protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTCGGTCATTCTCGCGGACTATGCACAA |
| Internal bar code: | CATTTGCGTCCTAGCCTCTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 688 |
| LEAP-Seq percent confirming: | 98.6836 |
| LEAP-Seq n confirming: | 11470 |
| LEAP-Seq n nonconfirming: | 153 |
| LEAP-Seq n unique pos: | 134 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATTGTGGTTGCTTGACT |
| Suggested primer 2: | CAAGCCCCAGGAATAGATCA |