Insertion junction: LMJ.RY0402.237439_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.237439
Chromosome:chromosome 3
Location:4890649
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g179450 SRR7 (1 of 1) K09624 - protease, serine, 12 (neurotrypsin, motopsin) [EC:3.4.21.-] (PRSS12); Scavenger receptor cysteine rich (SRCR) protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCACCTCCCTTCACACGCTCGCTTCTCTCC
Internal bar code:TGCGCCGCCCGGGTACAACACC

Confirmation - LEAP-Seq

LEAP-Seq distance:553
LEAP-Seq percent confirming:94.2381
LEAP-Seq n confirming:4465
LEAP-Seq n nonconfirming:273
LEAP-Seq n unique pos:75

Suggested primers for confirmation by PCR

Suggested primer 1:CTAAATGTGTAGCCAGCGCA
Suggested primer 2:CGGGCAGGAGTAGAAGAGTG