Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.237483 |
Chromosome: | chromosome 10 |
Location: | 1333284 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCTTCCTACCCGAACAGTGAATACCGCT |
Internal bar code: | GGCGCCCAGACTCGACGACTTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 605 |
LEAP-Seq percent confirming: | 94.5262 |
LEAP-Seq n confirming: | 3212 |
LEAP-Seq n nonconfirming: | 186 |
LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGGAAGTGCCCAGCTACAG |
Suggested primer 2: | TCATACCACCAGATTTGCGA |