Insertion junction: LMJ.RY0402.237483_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.237483
Chromosome:chromosome 10
Location:1333284
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCTTCCTACCCGAACAGTGAATACCGCT
Internal bar code:GGCGCCCAGACTCGACGACTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:605
LEAP-Seq percent confirming:94.5262
LEAP-Seq n confirming:3212
LEAP-Seq n nonconfirming:186
LEAP-Seq n unique pos:67

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGAAGTGCCCAGCTACAG
Suggested primer 2:TCATACCACCAGATTTGCGA