| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.237487 |
| Chromosome: | chromosome 12 |
| Location: | 2686338 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503950 | (1 of 10) PTHR10174 - RETINALDEHYDE BINDING PROTEIN-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCCACGGCGCTTTCGAGGTGTGATACCA |
| Internal bar code: | GACAACTGGGTCCCTCACAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 678 |
| LEAP-Seq percent confirming: | 97.2928 |
| LEAP-Seq n confirming: | 4061 |
| LEAP-Seq n nonconfirming: | 113 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACAGCAAGCAGGTAGGGC |
| Suggested primer 2: | AAGCTTTGCGAGCTTTTCTG |