| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.237503 |
| Chromosome: | chromosome 1 |
| Location: | 5286700 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036950 | CBA1 | (1 of 1) 2.7.8.26 - Adenosylcobinamide-GDP ribazoletransferase / Cobalamin-5'-phosphate synthase; Cobalamin 5'-phosphate synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTGCATCTCACACTTGCATCCCAAACC |
| Internal bar code: | CGACAGGCGGGCTAGTAACGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 661 |
| LEAP-Seq percent confirming: | 99.0022 |
| LEAP-Seq n confirming: | 4564 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTTCTCCCGGAATCCTGT |
| Suggested primer 2: | TGGCAGGTTGGAGATGTGTA |