| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237506 |
| Chromosome: | chromosome 17 |
| Location: | 3998001 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728900 | FSA1,TAL3 | (1 of 1) PTHR10683//PTHR10683:SF2 - TRANSALDOLASE // SUBFAMILY NOT NAMED; Transaldolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGGGGCACGGGGCCGCCTGCACGTGCC |
| Internal bar code: | TGTTTACTTCTCTCATGTGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1063 |
| LEAP-Seq percent confirming: | 99.4059 |
| LEAP-Seq n confirming: | 7864 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAGGGCAAACACGCTAAC |
| Suggested primer 2: | ATGGCTGTTTCCCTTGACAC |