| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237530 |
| Chromosome: | chromosome 1 |
| Location: | 119129 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g000900 | CPLD20 | Conserved in the Plant Lineage and Diatoms; (1 of 2) PF07082 - Protein of unknown function (DUF1350) (DUF1350) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTTGGTGTCGTCCAGCGAGTCGTCCTT |
| Internal bar code: | TCAATCAACTTATGTTACTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 431 |
| LEAP-Seq percent confirming: | 99.2954 |
| LEAP-Seq n confirming: | 8174 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCAGTGCAGGTACAAATG |
| Suggested primer 2: | ATAGGGACACGCAAGACCAC |