| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237552 |
| Chromosome: | chromosome 17 |
| Location: | 4280952 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g730750 | (1 of 1) PTHR10913:SF47 - FOLLISTATIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGATCCGCTATCTGGTGGCTAAGAAGGCG |
| Internal bar code: | ATTCGCTCTTGCGGTCCTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1080 |
| LEAP-Seq percent confirming: | 99.3885 |
| LEAP-Seq n confirming: | 2438 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGTAGGGACGGTGGAATA |
| Suggested primer 2: | CGTGGAGTACCCGAATGAGT |