| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.237592 |
| Chromosome: | chromosome 17 |
| Location: | 3622543 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g726150 | (1 of 1) PTHR23073:SF29 - P-LOOP CONTAINING NUCLEOSIDE TRIPHOSPHATE HYDROLASES SUPERFAMILY PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCGGCGGCGCCGCGGGCGGGCTGCCGG |
| Internal bar code: | CGCCCGCGCGTAATGAGATAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 596 |
| LEAP-Seq percent confirming: | 95.1952 |
| LEAP-Seq n confirming: | 3487 |
| LEAP-Seq n nonconfirming: | 176 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAGCAAGAAGGGACAAG |
| Suggested primer 2: | GCGTCAAGGCGAAGTAACTC |