| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.237604 |
| Chromosome: | chromosome_2 |
| Location: | 5742886 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g110350 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCTGCACAAAGCCGTGCTGCTTGCCCGACT |
| Internal bar code: | AGGACGGGCGGCATACATATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 884 |
| LEAP-Seq percent confirming: | 99.0745 |
| LEAP-Seq n confirming: | 1927 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAAGCATGTGTCAGATCA |
| Suggested primer 2: | CGGGTTCCTGACACAAGTTT |