| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.237610 |
| Chromosome: | chromosome_9 |
| Location: | 2265734 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g392653 | sense | 5'UTR|intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTTTTAACAAACAGGCGCTGCAATCAATC |
| Internal bar code: | AAGCCGCTCAACGCTCTGTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 522 |
| LEAP-Seq percent confirming: | 98.6243 |
| LEAP-Seq n confirming: | 1864 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTAAGGGTAACCTGCTG |
| Suggested primer 2: | GGTGCGTGAGTGTAATCGTG |