| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.237631 |
| Chromosome: | chromosome 14 |
| Location: | 3352802 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630100 | RPL13 | Ribosomal protein L13, component of cytosolic 80S ribosome and 6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCTGCACAACCCAAGCCCGCATCCCGC |
| Internal bar code: | ACCGCGAAACCAAAATTGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 266 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 103 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCCATGAGCGTTAAGGAG |
| Suggested primer 2: | GTTATTAGGGGGTCGGGTGT |