| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237644 |
| Chromosome: | chromosome 10 |
| Location: | 901615 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424250 | BUG13,FAP90 | Flagellar Associated Protein 90 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGAATGTGCGACGGCCTTTCAGTGGCTGC |
| Internal bar code: | TCGTGATAATAGGACTTTGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 978 |
| LEAP-Seq percent confirming: | 99.2704 |
| LEAP-Seq n confirming: | 4626 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGATGAGCAGGACTGGGT |
| Suggested primer 2: | CGGTAGAGGCACCTGTAGGA |