| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237645 |
| Chromosome: | chromosome 9 |
| Location: | 2646750 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390100 | SMM34 | (1 of 1) 2.1.1.103 - Phosphoethanolamine N-methyltransferase; S-adenosyl-L-methionine-dependent methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGTAGGCACGCCCCGCATTTCCGCCCTA |
| Internal bar code: | CGGGAGAATCCATTCTTGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 508 |
| LEAP-Seq percent confirming: | 99.6231 |
| LEAP-Seq n confirming: | 3436 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTCGAACGGATAGTGGTG |
| Suggested primer 2: | AATCTGCGTGGTGTTAAGGG |