| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.237649 |
| Chromosome: | chromosome 12 |
| Location: | 3606813 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513900 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCCTTCTTCTTCTTGTCCGCGCCGCCAA |
| Internal bar code: | GGCAAGGATGCAAAACTTTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 466 |
| LEAP-Seq percent confirming: | 99.0724 |
| LEAP-Seq n confirming: | 3738 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAAGTGAAGGCGTAGGCA |
| Suggested primer 2: | CTGCAATGCTGGTGCTTTTA |