| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.237667 |
| Chromosome: | chromosome 3 |
| Location: | 2778132 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g161976 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCTGGCATCGACCAAGCTACGGGCACTA |
| Internal bar code: | AAAAGTCCACACTGGCTTGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 895 |
| LEAP-Seq percent confirming: | 99.3964 |
| LEAP-Seq n confirming: | 13009 |
| LEAP-Seq n nonconfirming: | 79 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCAGCTCACCAAAATGAG |
| Suggested primer 2: | CGTGTCATACCCAGCATACG |