| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237682 |
| Chromosome: | chromosome_17 |
| Location: | 3085576 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g721000 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGTTCCCCAGGGTTCGCTTCTGGCGCAGT |
| Internal bar code: | ACCCGGTAGAAACACCGCGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1248 |
| LEAP-Seq percent confirming: | 99.3976 |
| LEAP-Seq n confirming: | 6600 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGCAGTTTGTGTTTGGG |
| Suggested primer 2: | GGTTCAGCAGGAGCTTGTTC |