| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.237688 |
| Chromosome: | chromosome 3 |
| Location: | 6285038 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193000 | HAD5 | Halo-acid dehalogenase-like hydrolase; (1 of 1) 3.1.3.29 - N-acylneuraminate-9-phosphatase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTAGGTGTACACGTACCAGCAAATGTCGG |
| Internal bar code: | CGCTGTATTATTAGCCGATTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1093 |
| LEAP-Seq percent confirming: | 99.6212 |
| LEAP-Seq n confirming: | 40504 |
| LEAP-Seq n nonconfirming: | 154 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCATAGAGCGGGACTACGC |
| Suggested primer 2: | TGTGTGAGAACCTGCTGAGG |