| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.237691 |
| Chromosome: | chromosome 10 |
| Location: | 571876 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g421576 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGGAGTCACGGTGCCGCAGCTAGCCGC |
| Internal bar code: | GAGGCCTCGACAAGAGGGCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 725 |
| LEAP-Seq percent confirming: | 98.9025 |
| LEAP-Seq n confirming: | 6038 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 98 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGACTCCATGCTGAATGA |
| Suggested primer 2: | AAGATAATACCGCACGCACC |