| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.237699 |
| Chromosome: | chromosome 2 |
| Location: | 6216683 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g114001 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTGGGCAGCGCCTCCACCTGTAACCCC |
| Internal bar code: | GGCTACGCCTACTTGACATACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 304 |
| LEAP-Seq percent confirming: | 65.8333 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCCAACGTCAGTGTGAG |
| Suggested primer 2: | TGCAGACGTGACTTGTCCTC |