| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.237725 |
| Chromosome: | chromosome 12 |
| Location: | 2403083 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g507200 | (1 of 2) IPR000104//IPR003613 - Antifreeze protein, type I // U box domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAATCGAAATAAACCGGGATTCAGAAGC |
| Internal bar code: | AATATAGGTCGGATGACGTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 597 |
| LEAP-Seq percent confirming: | 93.9762 |
| LEAP-Seq n confirming: | 3479 |
| LEAP-Seq n nonconfirming: | 223 |
| LEAP-Seq n unique pos: | 123 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGTGGTCTGTGTGTGG |
| Suggested primer 2: | ATAACCCAACAAAACGCTGC |