| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.237730 |
| Chromosome: | chromosome 5 |
| Location: | 463014 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g232500 | FXL8 | (1 of 2) IPR000014//IPR011990 - PAS domain // Tetratricopeptide-like helical domain; FixL-like PAS domain protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATGCGTTGGCCGTCCGTACCGCCCACCCG |
| Internal bar code: | TACCTGGCTATGCCCTCAATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 87.5263 |
| LEAP-Seq n confirming: | 1663 |
| LEAP-Seq n nonconfirming: | 237 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCACCCACACATCTCTCAA |
| Suggested primer 2: | CGGTACCGTATCATCAGCCT |