| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.237749 |
| Chromosome: | chromosome 12 |
| Location: | 7210521 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g558450 | SPD1 | Spermidine synthase; (1 of 1) K00797 - spermidine synthase (speE, SRM) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCCCAAACTTTTATGCCCCCACAGCAAC |
| Internal bar code: | GGGGCATCGTCGTCTGTCAATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 558 |
| LEAP-Seq percent confirming: | 93.4033 |
| LEAP-Seq n confirming: | 14145 |
| LEAP-Seq n nonconfirming: | 999 |
| LEAP-Seq n unique pos: | 109 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTCCACCGACCTACTGT |
| Suggested primer 2: | TGAATCCTTTTCCCTTCACG |