| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.237769 |
| Chromosome: | chromosome 8 |
| Location: | 494391 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358850 | FAP386 | Flagellar Associated Protein 386 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAATCTTGCAAGCTTGCCGTGAAGCAACG |
| Internal bar code: | GGTGTGACGAGGTGCCCGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 619 |
| LEAP-Seq percent confirming: | 93.1158 |
| LEAP-Seq n confirming: | 2083 |
| LEAP-Seq n nonconfirming: | 154 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGGTACAGGCCTACAGGG |
| Suggested primer 2: | GCATTAACCCATCATCACCC |