| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237789 |
| Chromosome: | chromosome 10 |
| Location: | 5935132 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462150 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATCAAAGCCATATGCGTGGGGCGGCAGC |
| Internal bar code: | CCTACTATAACACACTCGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 356 |
| LEAP-Seq percent confirming: | 93.6 |
| LEAP-Seq n confirming: | 702 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTACTGCCACTTCCGTT |
| Suggested primer 2: | GCTGAAGAGGAGCAACCAAC |