| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.237789 |
| Chromosome: | chromosome 10 |
| Location: | 5935134 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462150 | CDS/5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTGTAGTGATGGAGGAGCCCTCACGGT |
| Internal bar code: | GTTTCGAGTCCCCTAGAGCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 458 |
| LEAP-Seq percent confirming: | 98.5945 |
| LEAP-Seq n confirming: | 1403 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTACTGCCACTTCCGTT |
| Suggested primer 2: | GCTGAAGAGGAGCAACCAAC |