| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237802 |
| Chromosome: | chromosome 1 |
| Location: | 3292741 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g020800 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGAATAGGAAGCGATACGTTCCTCAAGC |
| Internal bar code: | AGCTTACTGGCGAAGTAGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 238 |
| LEAP-Seq percent confirming: | 99.6047 |
| LEAP-Seq n confirming: | 756 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTGGGAAGACAGGTCAG |
| Suggested primer 2: | TTCAGTCTTCTTGAGGGCGT |