| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.237814 |
| Chromosome: | chromosome 13 |
| Location: | 4488586 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g602300 | TMC4 | (1 of 1) 2.1.1.202 - Multisite-specific tRNA:(cytosine-C(5))-methyltransferase; putative tRNA (cytosine-5)-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGACCGCGGCGGCTGGGGCCCCATCTAC |
| Internal bar code: | GACAGACCATGTGAACAGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 379 |
| LEAP-Seq percent confirming: | 59.5 |
| LEAP-Seq n confirming: | 119 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGGGTGCACTATGTGGG |
| Suggested primer 2: | CTGTTGTCACACAACCTGGG |