| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237818 |
| Chromosome: | chromosome 1 |
| Location: | 3355124 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g021200 | CGL47 | conserved protein with F-box domain; (1 of 10) IPR000104//IPR001810 - Antifreeze protein, type I // F-box domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTCTGTGGTCTGGCCCGACACCTGAGGG |
| Internal bar code: | GCCGTAAAGATAGCTCGTTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 661 |
| LEAP-Seq percent confirming: | 98.6842 |
| LEAP-Seq n confirming: | 7050 |
| LEAP-Seq n nonconfirming: | 94 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCCTTTTGAAATCGTGTA |
| Suggested primer 2: | CACCTGACCAGTCACCAATG |