| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237847 |
| Chromosome: | chromosome 3 |
| Location: | 4078385 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g172750 | (1 of 1) IPR002893//IPR012677 - Zinc finger, MYND-type // Nucleotide-binding alpha-beta plait domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAACCGCCTGGCGGCTCGCAAGGGAGCTG |
| Internal bar code: | ATGGACACTCCTGCAGGCGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1014 |
| LEAP-Seq percent confirming: | 99.3778 |
| LEAP-Seq n confirming: | 2236 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCATCAAGTCAAGCAGTC |
| Suggested primer 2: | GTGTCGACGAATTGCTGAGA |