| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237871 |
| Chromosome: | chromosome 3 |
| Location: | 7277263 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207200 | (1 of 18) PF00650 - CRAL/TRIO domain (CRAL_TRIO) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTCTCATTAATCATACGCCTTCAGCCCG |
| Internal bar code: | TCGTTAGCTTTAGAGACATCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 179 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAGTTGAGGCAGGCAGG |
| Suggested primer 2: | TGCAATTTTCACAGAGCTGG |