| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.237875 |
| Chromosome: | chromosome_5 |
| Location: | 878171 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g247600 | UBC2 | Ubiquitin-conjugating enzyme E2 | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGAGTAACGTCGAAACAAAGCTAGCTGCGA |
| Internal bar code: | CTGGATCGAGCGTGGGCCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 268 |
| LEAP-Seq percent confirming: | 17.9 |
| LEAP-Seq n confirming: | 179 |
| LEAP-Seq n nonconfirming: | 821 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCTCTAATGCTTCACACA |
| Suggested primer 2: | AAAATGGAGGATTTTTGGGG |