| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.237905 |
| Chromosome: | chromosome 3 |
| Location: | 1752583 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g153850 | (1 of 10) PTHR31867:SF1 - EXPANSIN-A1-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCAGTCCCTTCTGGTGCAGTTTTACCG |
| Internal bar code: | GTACGTGACAGTTAAAACGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 750 |
| LEAP-Seq percent confirming: | 99.0128 |
| LEAP-Seq n confirming: | 1003 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAACATGCTTCGCTTACG |
| Suggested primer 2: | TGGGTTCTCATCTCTCGCTT |