| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237911 |
| Chromosome: | chromosome 12 |
| Location: | 3934444 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g516300 | CGL154 | Conserved in the Green Lineage; (1 of 11) PF13229 - Right handed beta helix region (Beta_helix) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGTCCTGACGCGCCGCCCATGGGCCTGC |
| Internal bar code: | CGCCTAGGTGATTTATAGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 310 |
| LEAP-Seq percent confirming: | 99.5862 |
| LEAP-Seq n confirming: | 5535 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGCAAACCCATAATGCAA |
| Suggested primer 2: | AATTACTGGCTGAACGTGCC |