| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.237913 |
| Chromosome: | chromosome 10 |
| Location: | 887646 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424050 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCAGGGGCTAAAGATGCGGCAAGAATGC |
| Internal bar code: | TTGTATTAATTTATGATGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 597 |
| LEAP-Seq percent confirming: | 99.194 |
| LEAP-Seq n confirming: | 7384 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCACTGTCCGCAAACTCA |
| Suggested primer 2: | CCCGTACTCTGCATTGAACA |