| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.237916 |
| Chromosome: | chromosome 14 |
| Location: | 1464273 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g618000 | EFH9 | EF-hand Calcium binding protein; (1 of 5) PTHR23050:SF90 - CENTRIN-3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGATGTCGTCCGTGGGGTTCTGCGTGCCG |
| Internal bar code: | GCCCGCTGCACGAGCGACATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 246 |
| LEAP-Seq percent confirming: | 98.5401 |
| LEAP-Seq n confirming: | 135 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTTACCATGAACGGCTTT |
| Suggested primer 2: | ATTTCCGCCAACAAACTCAC |