| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237923 |
| Chromosome: | chromosome 2 |
| Location: | 5655104 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109650 | GSP1 | Gamete-specific plus 1; (1 of 2) IPR001356//IPR008422//IPR009057 - Homeobox domain // Homeobox KN domain // Homeodomain-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGAAGACGAGCAGCCGCTTGAGCAGGGG |
| Internal bar code: | GTTCTCATTACTACAGATGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1115 |
| LEAP-Seq percent confirming: | 99.5011 |
| LEAP-Seq n confirming: | 21741 |
| LEAP-Seq n nonconfirming: | 109 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCTTGCACGAATCAGTC |
| Suggested primer 2: | TGTGGTGGGTACTGCTGGTA |