| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.237926 |
| Chromosome: | chromosome 2 |
| Location: | 6750769 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g118450 | DNJ28 | (1 of 1) IPR000104//IPR001623//IPR012336 - Antifreeze protein, type I // DnaJ domain // Thioredoxin-like fold; DnaJ-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAGCGAGCTGGCGGCGGGCGGCGAGGAC |
| Internal bar code: | ATTGCGTTGACGATATCAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 885 |
| LEAP-Seq percent confirming: | 86.6345 |
| LEAP-Seq n confirming: | 4129 |
| LEAP-Seq n nonconfirming: | 637 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCCTCCTTCAGGACTTT |
| Suggested primer 2: | TTACTGCATCGCAGCATACC |