Insertion junction: LMJ.RY0402.237977_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.237977
Chromosome:chromosome 3
Location:2269526
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g157950 (1 of 1) IPR011989//IPR011990//IPR016024 - Armadillo-like helical // Tetratricopeptide-like helical domain // Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGTTACGTCGATATTAATTGGATACAGTG
Internal bar code:GAGGACTGGACTCTCTCGCCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:635
LEAP-Seq percent confirming:98.885
LEAP-Seq n confirming:17648
LEAP-Seq n nonconfirming:199
LEAP-Seq n unique pos:131

Suggested primers for confirmation by PCR

Suggested primer 1:GCCATGTCACAGTCCAACAC
Suggested primer 2:TCGGTGTGTCAATGTGTGTG