Insertion junction: LMJ.RY0402.237978_4

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.237978
Chromosome:scaffold 19
Location:60527
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre19.g750447 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTCATAACGCAGATCACGCGATGCATCG
Internal bar code:TTGGCGTGTTGGATTTCAGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:272
LEAP-Seq percent confirming:97.473
LEAP-Seq n confirming:8486
LEAP-Seq n nonconfirming:220
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:TACACCGCACCAAATACCAA
Suggested primer 2:COULD_NOT_FIND_PRIMER