Insertion junction: LMJ.RY0402.237991_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.237991
Chromosome:chromosome 16
Location:1751900
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g654950 SRR3 Scavenger receptor cysteine rich (SRCR) protein; (1 of 18) PTHR19862:SF14 - WD REPEAT-CONTAINING PROTEIN 48 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCTACGTTGGCGCCAAAGTAGGACCGGC
Internal bar code:GAGAATCGGCTCGGCGCGCATC

Confirmation - LEAP-Seq

LEAP-Seq distance:225
LEAP-Seq percent confirming:89.2031
LEAP-Seq n confirming:694
LEAP-Seq n nonconfirming:84
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GTATGGTCAATGGTCAGGGG
Suggested primer 2:CAGGTTGGTTGGATGGATTC