Insertion junction: LMJ.RY0402.237999_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.237999
Chromosome:chromosome 6
Location:5458877
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g284450 PPP24 (1 of 2) K15731 - carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase [EC:3.1.3.16] (CTDSP); Phosphoprotein phosphatase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTAGGTCAGTCAGGTAGTCAAACGCACGT
Internal bar code:GACCCGCGTTCTAAACACATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1062
LEAP-Seq percent confirming:99.2258
LEAP-Seq n confirming:769
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AGAATGGTTGCACAAGGGAC
Suggested primer 2:CATGCCCGCAGTAGTATGTG