| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.238005 |
| Chromosome: | chromosome 11 |
| Location: | 2709841 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACTAGCGAACATGTGTTTGGTTTTTTTT |
| Internal bar code: | AGGCGTTCGGAACTCAGAACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 63 |
| LEAP-Seq percent confirming: | 96.8535 |
| LEAP-Seq n confirming: | 14806 |
| LEAP-Seq n nonconfirming: | 481 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACCATTCTACCGCAACT |
| Suggested primer 2: | CTACGCTTAGCGACCAAAGG |