Insertion junction: LMJ.RY0402.238018_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.238018
Chromosome:chromosome 3
Location:6300306
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g193250 (1 of 1) 2.7.1.160 - 2'-phosphotransferase / Yeast 2'-phosphotransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAGGACCTTTCCCTCCTGGCGATCAGGCC
Internal bar code:GTATTGCATACATTGGGGAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:643
LEAP-Seq percent confirming:98.7185
LEAP-Seq n confirming:2465
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:GCACCTTCTCAGTACTCGCC
Suggested primer 2:TCAGCTTCGAACCTTGACCT